Article Text

PDF

Peripheral CD25 positive T lymphocytes with biased T cell receptor Vβ gene usage in autoimmune endogenous posterior uveitis
  1. P J Tighe,
  2. J V Forrester,
  3. J Liversidge,
  4. H F Sewell
  1. Department of Immunology, Faculty of Medicine, University Hospital Medical School, Nottingham NG7 2UH
  2. Department of Ophthalmology, University of Aberdeen Medical School, Aberdeen AB9 2ZD

    Abstract

    Aims—To determine T cell receptor (TCR) Vβ gene usage in peripheral blood T lymphocytes of patients with endogenous posterior uveitis (EPU). If biased TCR variable (V) gene usage occurs in this autoimmune disease, it should be detectable in immune activated peripheral blood T cells in vivo.

    Methods—Relative proportions of each Vβ gene family expressed in total peripheral blood lymphocytes (PBL) and in vivo activated (CD25+) T cells from patients with EPU and controls were determined using the anchored polymerase chain reaction (anchored PCR) in conjunction with a novel hybridisation assay. The TCR Vβ repertoires seen in these cell populations were then compared.

    Results—Vβ1 usage within the CD25+ lymphocytes of patients with EPU was substantially elevated (mean ± SD 15 ±9%) compared with control CD25+ cells (3.3 ±2.4%).

    Conclusions—By contrasting the repertoires of these cell populations, biased TCR Vβ gene usage was detected in patients with EPU, namely increased usage of Vβ1 in CD25+ T cells from peripheral blood of these patients. This approach of directly analysing the activated T cells in blood, using bulk PBL as an internal control, has wide applicability where specific T cell subpopulations are thought to play an important aetiopathological role.

    • T cell receptor
    • Vβ gene
    • endogenous posterior uveitis

    Statistics from Altmetric.com

    Request permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.