Article Text

PDF

Establishment and characterisation of two cell lines derived from a primary adenocarcinoma of the duodenum
  1. M Golding,
  2. G W H Stamp,
  3. T Oates,
  4. E-N Lalani
  1. Department of Histopathology, Royal Postgraduate Medical School, Du Cane Road, London W12 0NN

    Abstract

    Aims—To establish two cell lines from a primary duodenal adenocarcinoma; to describe the morphological, growth, ploidy, and immunophenotypic characteristics of these cell lines.

    Methods—The cell lines, designated DAC/S and DAC/E, were characterised using both in vitro and in vivo cell culture techniques, light and electron microscopy, immunocytochemistry, and FACS analyses.

    Results—Both cell lines have an epithelial origin, are aneuploid and display characteristics of transformed cells. The cell lines differ from each other in morphology, doubling time and serum requirements. These cell lines are anchorage dependent and do not grow in nude mice.

    Conclusions—DAC/S and DAC/E cell lines are derived from neoplastic epithelium and could provide in vitro model systems for future investigations of the cell and molecular biology of duodenal neoplasia.

    • duodenum
    • adenocarcinoma
    • cell lines
    • integrins
    • intermediate filaments

    Statistics from Altmetric.com

    Request permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.