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Effect of Wnt-1 antisense RNA on the outgrowth of a mammary adenocarcinoma cell line expressing that oncogene
  1. J Polanec,
  2. Z P Pavelic,
  3. W L Myers
  1. Department of Medical Microbiology and Immunology, Southern Illinois University School of Medicine, PO Box 19230, Springfield, IL 62794-1220, USA
  2. Department of Otolaryngology-Head and Neck Surgery, College of Medicine, University of Cincinnati, 231 Bethesda Avenue, Cincinnati, Ohio 45267-0528, USA

    Abstract

    Aims—To investigate the effect of Wnt-1 antisense RNA on the outgrowth of a mammary tumour cell line expressing that oncogene.

    Methods—A plasmid (pMT 70), containing Wnt-1 cDNA, was cut with appropriate enzymes and inserted into a eukaryotic expression vector (pMAMneo). A mammary tumour cell line (CAC-L153) was transfected with the expression vector and cells with the vector in sense and antisense orientation were selected.

    Results—Tumour cells with the expression vector in the antisense orientation had a notable reduction in expression of Wnt-1 protein and a considerable reduction in tumour outgrowth compared with controls.

    Conclusions—The results indicate that the Wnt-1 proto-oncogene may be a possible target for antisense therapy.

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