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A rapid RT-PCR based method for the detection of BCR-ABL translocation.
  1. Sidorova JYu,
  2. L B Saltykova,
  3. A A Lyschov,
  4. Zaritskey AYu,
  5. K M Abdulkadyrov,
  6. M N Blinov
  1. Institute of Haematology and Transfusiology, St Petersburg, Russia.

    Abstract

    AIMS: To optimise a one step reverse transcriptase polymerase chain reaction (RT-PCR) protocol for BCR-ABL chimaera detection. METHODS: Compared with published RT-PCR procedures, this novel approach has at least two advantages. First, the same enzyme is used for both reverse transcription and PCR. Second, amplification of the target (BCR-ABL chimaera) and control gene (ABL) is performed simultaneously in the same tube. RESULTS: On testing 40 chronic myelogenous leukaemia patients and 10 healthy donors there was a specificity for the newly developed technique. In addition, dilution experiments demonstrated that the protocol was highly sensitive. CONCLUSIONS: The suggested one step PCR strategy is a simple and reliable way to reveal BCR-ABL chimaeras.

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