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Cloning of a mRNA Preferentially Expressed in Chondrocytes by Differential Display-PCR from a Human Chondrocytic Cell Line That Is Identical with Connective Tissue Growth Factor (CTGF) mRNA,☆☆

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Abstract

Chondrocyte- or chondrosarcoma cell line (HCS)-specific DNA fragments were obtained using differential display-PCR. Nucleotide sequences of 32 species derived from HCS cells were determined. One of the sequence tags (tag no. 24) corresponded to the nucleotide sequence of connective tissue growth factor (CTGF). Northern blot analysis showed that CTGF was highly expressed in HCS cells and rabbit growth cartilage cells in culture but was not expressed in osteoblastic cells in culture.In situhybridization revealed that CTGF was expressed only in the hypertrophic chondrocytes of costal cartilage and the vertebral column in embryonic mice. The expression of CTGF in HCS cells was up-regulated by the addition of TGF-β or BMP-2. These findings suggest that CTGF participates in endochondral ossification.

References (35)

  • M. Iwamoto et al.

    Exp. Cell Res.

    (1993)
  • R. Lobb et al.

    J. Biol. Chem.

    (1986)
  • Y. Kato et al.

    J. Biol. Chem.

    (1990)
  • S.C. Chang et al.

    J. Biol. Chem.

    (1994)
  • Y. Ohba et al.

    Biochim. Biophys. Acta

    (1995)
  • P. Bork

    FEBS Lett.

    (1993)
  • S. Noji et al.

    FEBS Lett.

    (1989)
  • M. Iwamoto et al.

    J. Biol. Chem.

    (1991)
  • A.R. Poole

    Cartilage: Molecular Aspects

    (1991)
  • M. Takigawa et al.

    Proc. Natl. Acad. Sci. USA

    (1980)
  • M. Takigawa et al.

    J. Cell. Physiol.

    (1981)
  • E. Schipani et al.

    Science

    (1995)
  • M. Takigawa et al.

    Endocrinology

    (1988)
  • M. Enomoto et al.
  • M. Enomoto et al.

    J. Biochem.

    (1990)
  • S.T. Trippel
  • M. Noda et al.

    Endocrinology

    (1989)
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    IGF, insulin-like growth factorFGF, fibroblast growth factor; TGF, transforming growth factor; BMP, bone morphological protein; PCR, polymerase chain reaction; RT-PCR, reverse transcriptase-PCR; HCS, human chondrosarcoma-derived chondrocyte; CTGF, connective tissue growth factor; MEM, minimum essential medium; DMEM, Dulbecco's minimum essential medium; ALPase, alkaline phosphatase; RGC, rabbit growth cartilage; RRC, rabbit resting cartilage;

    ☆☆

    B. HallS. Newman, Eds.

    1

    To whom correspondence should be addressed at Department of Biochemistry and Molecular Dentistry, Okayama University Dental School, 2-5-1 Shikata-cho, Okayama 700, Japan. Fax: +81-86-222-0154. E-mail: [email protected].

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