Evidence for a third transcript from the human factor VIII gene*
References (25)
- et al.
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
Anal. Biochem.
(1987) - et al.
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
Anal. Biochem.
(1983) - et al.
Synthetic factor VIII peptides with amino acid sequences contained within the C2 domain of factor VIII inhibit factor VIII binding to phosphotidylserine
Blood
(1990) - et al.
Processing of the platelet-derived growth factor receptor
J. Biol. Chem.
(1987) - et al.
The sequence of the human factor VIII associated gene is conserved in mouse
Genomics
(1992) - et al.
A transcribed gene in an intron of the human factor VIII gene
Genomics
(1990) - et al.
Localization of epitopes for human factor VIII inhibitor antibodies by immunoblotting and antibody neutralization
Blood
(1989) - et al.
Molecular basis of factor VIII inhibition by human antibodies: Antibodies that bind to the factor VIII light chain prevent the interaction of factor VIII with phospholipid
J. Clin. Invest.
(1989) - et al.
α1(IV) and α2(IV) collagen genes are regulated by a bidirectional promoter and a shared enhancer
- et al.
Laboratory methods: A method for isolation of intact translationally active ribonucleic acid
DNA
(1983)
Characterization of the human factor VIII gene
Nature
Detection and sequence of mutations in the factor VIII gene of haemophiliacs
Nature
Cited by (98)
Race, ethnicity, F8 variants, and inhibitor risk: analysis of the “My Life Our Future” hemophilia A database
2023, Journal of Thrombosis and HaemostasisRisk factors for inhibitor development in severe hemophilia a
2018, Thrombosis ResearchEngineering less immunogenic and antigenic FVIII proteins
2016, Cellular ImmunologyHunting down factor VIII in the immunopeptidome
2016, Cellular ImmunologyCitation Excerpt :Nevertheless, low-level expression of FVIII in other tissues cannot be excluded. In this respect it is interesting to note that low levels of F8 and F8a mRNA (but not F8b which is not expressed in mouse tissues [20]) have been detected in murine medullary thymic endothelial cells (mTEC) [21], which are responsible for negative selection of auto-reactive T cells in the thymus [22]. This raises the possibility that (1) FVIII is expressed in the mTEC of severe hemophilia A patients with the intron 22 inversion and (2) that consequently the immunological repertoire of these patients is not expected to contain CD4+ T cells directed towards FVIII.
The intron-22-inverted F8 locus permits factor VIII synthesis: Explanation for low inhibitor risk and a role for pharmacogenomics
2015, BloodCitation Excerpt :Because the I22I arises through a homologous recombination event that restores completely the sequence within which it occurs—which includes the promoter and first exon of the nested F8B gene—transcription and translation of F8B is expected to be unaffected (ie, wild type).5,22 The F8B locus encodes a widely expressed, moderately abundant 2.6-kb transcript with exons 23 to 26 of F8 spliced in-frame to an unrelated first exon that has a Kozak's consensus initiation codon.22 The F8B mRNA is predicted to encode a 216-amino-acid protein, FVIIIB, corresponding to exons 23 to 26 in F8.
Plasma Components as Targets of Damage
2014, Pathobiology of Human Disease: A Dynamic Encyclopedia of Disease Mechanisms
- *
The sequence of the cDNA reported in this article has been deposited with GenBank under Accession No. M90707.
- 1
Present address: Sanofi Diagnostics Pasteur, Inc., Chaska, MN 55318.