PT  - JOURNAL ARTICLE
AU  - P Rieske
AU  - J Bartkowiak
AU  - A Szadowska
AU  - M Debiec-Rychter
TI  - Malignant fibrous histiocytomas and H-ras-1 oncogene point mutations.
AID  - 10.1136/mp.52.2.64
DP  - 1999 Apr 01
TA  - Molecular Pathology
PG  - 64--67
VI  - 52
IP  - 2
4099  - http://mp.bmj.com/content/52/2/64.short
4100  - http://mp.bmj.com/content/52/2/64.full
SO  - Mol Pathol1999 Apr 01; 52
AB  - AIMS: To investigate the types and the frequencies of H-ras-1 gene mutations in malignant fibrous histiocytomas. METHODS: Thirty five samples of malignant fibrous histiocytoma tissue were searched for point mutations within "hot spot" codons 12 and 13 of the H-ras-1 oncogene by the specific "nested" polymerase chain reaction followed by restriction fragment length polymorphism (PCR-RFLP) and a direct cycle sequencing procedure. RESULTS: In contrast to previous reports, none of the tumours contained a point mutation or any other changes within or around the hot spot gene sequences. CONCLUSIONS: These data indicate that H-ras-1 oncogenic activation is not required in the molecular pathway of malignant fibrous histiocytoma formation and cannot be used as a discriminating factor for diagnostic sarcoma typing.