RT Journal Article
SR Electronic
T1 Malignant fibrous histiocytomas and H-ras-1 oncogene point mutations.
JF Molecular Pathology
JO Mol Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 64
OP 67
DO 10.1136/mp.52.2.64
VO 52
IS 2
A1 P Rieske
A1 J Bartkowiak
A1 A Szadowska
A1 M Debiec-Rychter
YR 1999
UL http://mp.bmj.com/content/52/2/64.abstract
AB AIMS: To investigate the types and the frequencies of H-ras-1 gene mutations in malignant fibrous histiocytomas. METHODS: Thirty five samples of malignant fibrous histiocytoma tissue were searched for point mutations within "hot spot" codons 12 and 13 of the H-ras-1 oncogene by the specific "nested" polymerase chain reaction followed by restriction fragment length polymorphism (PCR-RFLP) and a direct cycle sequencing procedure. RESULTS: In contrast to previous reports, none of the tumours contained a point mutation or any other changes within or around the hot spot gene sequences. CONCLUSIONS: These data indicate that H-ras-1 oncogenic activation is not required in the molecular pathway of malignant fibrous histiocytoma formation and cannot be used as a discriminating factor for diagnostic sarcoma typing.