Table 3

Optimised ARMS conditions for 11 tumours and the T47D cell line with a p53 mutation

Patient/cell line	3′ mismatch	2nd mismatch	Product size	Ta (°C)	Mg conc. (mM)	No. of PCR cycles
*ARMS failed to optimise.
ARMS, amplification refractory mutation system; Ta, annealing temperature.
T47D	T–G	A–A	127 bp	64	1.75	35
B3	A–G	A–A	87 bp	64	2	35
B11	G–T	C–T	140 bp	67	1.5	35
B16	G–G	C–C	130 bp	64	2	35
B27	A–C	A–G	143 bp	60	2	35
B28*	G–T	G–G	78 bp
B30	T–C	C–C	115 bp	56	2	35
B33*	G–T	G–G	78 bp
B37	A–C	C–T	112 bp	69	1.75	33
B45	T–G	C–C	125 bp	60	1.75	35
B46*	A–G	C–T	170 bp
B50*	G–T	T–C	170 bp