Primers used to amplify exons 5–8 of the p53 gene using nested PCR
| Exon | Primers | Length of PCR product | Annealing temperature |
|---|---|---|---|
| Set 1 (external primers) | |||
| 5 | F: 5′ GTTTCTTTGCTGCCGTGTTC 3′ | 313 bp | 64°C |
| R: 5′ ACCCTGGGCAACCAGCCCTGT 3′ | |||
| 6 | F: 5′ TGGTTGCCCAGGGTCCCCAG 3′ | 223 bp | 64°C |
| R: 5′ GGAGGGCCACTGACAACCA 3′ | |||
| 7 | F: 5′ CTTGCCACAGGTCTCCCCAA 3′ | 237 bp | 64°C |
| R: 5′ AGGGGTCAGCGGCAAGCAGA 3′ | |||
| 8 | F: 5′ TTGGGAGTAGATGGAGCCT 3′ | 281 bp | 62°C |
| R: 5′ AGGCATAACTGCACCCTTGG 3′ | |||
| Set 2 (internal primers) | |||
| 5 | F: 5′ TTCCTCTTCCTACAGTACTC 3′ | 214 bp | 55°C |
| R: 5′ GCCCCAGCTGCTCACCATCG 3′ | |||
| 6 | F: 5′ GGCCTCTGATTCCTCACTGATT 3′ | 167 bp | 57°C |
| R: 5′ AGAGACCCCAGTTGCAAACC 3′ | |||
| 7 | F: 5′ AGGCGCACTGGCCTCATCTT 3′ | 177 bp | 58°C |
| R: 5′ TGTGCAGGGTGGCAAGTGGC 3′ | |||
| 8 | F: 5′ TGCTTCTCTTTTCCTATCCTGA 3′ | 186 bp | 60°C |
| R: 5′ CGCTTCTTGTCCTGCTTGCT 3′ | |||