Repeat length of individual DNA sequences subcloned from a PCR product pool after amplification of mononucleotide or dinucleotide repeat markers
| Locus | Repeat | Repeat length and corresponding number of clones | N | % Correct | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Each locus was amplified by PCR with either Taq or Pfu, as indicated. | |||||||||||||
| The number of clones found containing varying numbers of repeat units is shown, with the correct number of repeats for each locus shown in bold. | |||||||||||||
| N, total number of cloned inserts sequenced for each locus; % Correct, the percentage containing the exact repeat length. | |||||||||||||
| RAC1 | (T)9 | 9 | |||||||||||
| (Taq) | 10 | 10 | 100 | ||||||||||
| RAC1 | (T)11 | 10 | 11 | ||||||||||
| (Taq) | 1 | 9 | 10 | 90 | |||||||||
| Bat–13 | (T)13 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | |||||
| (Taq) | 9 | 8 | 20 | 19 | – | 1 | 1 | 58 | 33 | ||||
| (Pfu) | – | – | 2 | 16 | 1 | – | – | 19 | 84 | ||||
| Bat–21 | (T)21 | 16 | 17 | 19 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | ||
| (Taq) | 1 | 1 | 2 | 1 | – | 6 | 5 | 19 | 3 | – | 29 | 3 | |
| (Pfu) | – | – | – | – | 2 | 2 | 2 | 7 | 3 | 2 | 19 | 0 | |
| Bat–21 | (TA)11 | 6 | 9 | 10 | 11 | 12 | 13 | ||||||
| (Taq) | 1 | 2 | 21 | 2 | 1 | 2 | 29 | 7 | |||||
| (Pfu) | – | 4 | 12 | 3 | – | – | 19 | 16 | |||||
| Bat–21 | (T)21 + (TA)11 | 35 | 36 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | |||
| (Taq) | 1 | 1 | 1 | – | 10 | 5 | 8 | 2 | 1 | 29 | 34 | ||
| (Pfu) | – | – | – | 2 | 3 | 1 | 8 | 4 | 1 | 19 | 16 | ||
| Bat–26 | (A)26 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | ||
| (Taq) | – | 1 | 1 | 2 | 1 | 4 | 10 | 12 | 2 | 1 | 34 | 35 | |
| (Pfu) | 1 | 1 | 1 | 5 | 7 | 7 | 8 | 9 | – | 1 | 40 | 23 | |
| D15S128 | (CA)18 | 14 | 15 | 16 | 17 | 18 | |||||||
| (Taq) | – | 1 | 1 | 3 | 9 | 14 | 64 | ||||||
| (Pfu) | 2 | – | 2 | 6 | 5 | 15 | 33 | ||||||