Analysis of paraffin wax embedded bone marrow biopsies for c-kit mutation Asp 816 to Val
| Material | n | DNA amplification | c-kit mutation |
|---|---|---|---|
| c-kit mutation was determined by HinfI digestion and sequencing. | |||
| The controls comprised CMML without concomittant mastocytosis. | |||
| *1/5 with loss of heterozygosity (?). | |||
| MC, mast cells; WT, wild-type; CMML, chronic myelomonocytic leukaemia. | |||
| Patient | |||
| Total biopsy sections | 1 | 1/1 | 1/1 |
| MC infiltrates | 10 | 7/10 | 5*/7 |
| CMML infiltrates | 10 | 6/10 | 4/10 |
| Controls | |||
| Total biopsy sections | 20 | 20/20 | 0/20 |