What to spot: options available
| Source of clone | Spotted DNA | Region of interest | Primer for cDNA synthesis |
|---|---|---|---|
| SSH, suppression subtractive hybridisation. | |||
| Publicly available clones, such as those from the IMAGE consortium | Entire clone or entire plasmids | Region unknown/unspecified | Random hexamers |
| In house derived—for example, by SSH | PCR product from plasmid | Specific region | Specific primers |
| Custom made/purchased libraries | Expressed sequence tags | 3′ end of gene | Poly(T) |