Primers and probes for TaqMan PCR of HHV-8 ORF-26 and v-cyclin encoding regions
| Primer | Sequence |
|---|---|
| F, FAM; HHV-8, human herpesvirus 8; ORF, open reading frame; T, TAMRA; v-cyclin, viral cyclin. | |
| KS1 ORF-26 | 5′-AGC CGA AAG GAT TCC ACC AT-3′ |
| KS2 ORF-26 | 5′-TCC GTG TTG TCT ACG TCC AG-3′ |
| TaqMan probe ORF-26 | 5′-F-CGC TAT TCT GCA GCA GCT GTT GGT GTA CCA-T-3′ |
| v-Cyclin 1 | 5′-ACC AGT TCA CTT TGC TAT GCC-3′ |
| v-Cyclin 2 | 5′-GCT TTT GTA ATC AGG GTG TTG AC-3′ |
| TaqMan probe v-cyclin | 5′-F- CAG ACT CCT TTT CCC GCC AAG AAC TTA TAG-T-3′ |
| β-Actin forward primer | 5′-TCA CCC ACA CTG TGC CCA TCT ACG A-3′ |
| β-Actin reverse primer | 5′-CAG CGG AAC CGC TCA TTG CCA ATG G-3′ |
| TaqMan probe β actin | 5′-F-ATG CCC TCC CCC ATG CCA TCC TGC GT-T-3′ |