Differences in proteinase and inhibitor production and MMP activity in the different breast tissues (median (range))
| Breast tumours (n = 51) | Background breast (n = 43) | Normal breast (n = 10) | MCF-7 cells (n = 10) | |
|---|---|---|---|---|
| For proteinases and inhibitors, the values are arbitrary units (OD × area of band). | ||||
| For RSH (rate of substrate hydrolysis) the units are pM/min. | ||||
| MMP, matrix metalloproteinase; OD, optical density; TIMP, tissue inhibitor of metalloproteinases; uPA, urokinase-type plasminogen activator. | ||||
| *p < 0.05, ANOVA, Mann Whitney, tumour versus other breast tissues | ||||
| MMP-9 | ||||
| Latent | 0–33.6 (10.5) | 0–24.9 (6.6) | 0–12.6 (7.8) | 0–23.4 (4.5) |
| Active | 0–23.5 (11.6)* | 0–7.6 (0) | 0 | 0 |
| Total | 0–70.6 (12.0) | 0–24.7 (7.1) | 0–12.6 (7.8) | 0–23.4 (4.5) |
| MMP-2 | ||||
| Latent | 0.3–20.46 (5.7) | 0.2–18.9 (3.2) | 1.8–14.4 (6.8) | 3.3–26.3 (8.5) |
| Active | 0–34.2 (5.3)* | 0–11.8 (0) | 0–1.1 (0.25) | 0 |
| Total | 0.3–52.0 (8.3) | 0.2–12.1 (3.6) | 2.9–15.4 (7.1) | 3.3–26.3 (8.5) |
| MMP-3 | ||||
| Latent | 0–9.2 (5.0)* | 0–3.7 (0) | 0 | 0 |
| Active | 0–27.7 (0) | 0–17.6 (0) | 0–7.7 (2.13) | 0 |
| Total | 0–31.4 (4.0)* | 0–17.6 (0) | 0–7.7 (2.13) | 0 |
| MMP-1 | ||||
| Latent | 0–2.6 (0) | 0 (0) | 0 | 0 |
| Active | 0–3.3 (0) | 0 (0) | 0 | 0 |
| Total | 0–5.9 (0) | 0 (0) | 0 | 0 |
| uPA | 0–56.0 (6.9) | 0–16.0 (1.5) | 0–7.3 (5.2) | 0 |
| TIMP-1 | 0–224.2 (6.0) | 0–24.3 (1.6) | – | 0 |
| TIMP-2 | 3.1–36.5 (11.7) | 0–26.4 (7.7) | – | 0 |
| RSH | 0–8325.0 (808.4)* | 0–2245.0 (225.1) | 6.4–2763.6 (278.7) | 81.5–621.8 (468.2) |