Virus transfected cells | ||||||
---|---|---|---|---|---|---|
DLD-1* | 16Da | 16Db | CaSki | PC-14† | 16Pa | |
CaSki, CaSki cell infected with HPV-16; –, not detected; for HPV mRNA, +, ++, and +++: positive and the density of the band was similar to that for 10, 50, and 100 copies of HPV-16 in pBR322, respectively; in other columns, +, ++, and +++: positive at 1×, 10×, and 100× dilution of the sample, respectively. | ||||||
*Non-HPV transfected DLD-1 cells (control); †Non-HPV transfected PC-14 cells (control). | ||||||
HPV | ||||||
E1 | − | − | − | + | − | − |
E2 | − | +++ | − | + | − | − |
E4 | − | + | + | +++ | − | − |
E5 | − | + | − | + | − | + |
E6 | − | +++ | + | ++ | − | + |
E7 | − | +++ | − | + | − | + |
L1 | − | − | − | +++ | − | − |
L2 | − | − | − | − | − | + |
E6–E7 | − | +++ | − | − | − | |
E6–E2 | − | − | − | − | − | |
E6–L1 | − | − | − | − | ||
Bax | + | + | + | − | ++ | |
Bcl2 | + | + | + | ++ | ++ | |
Fas | + | + | + | ++ | ++ | |
Fas L | − | − | − | − | − | |
DNase γ | + | ++ | + | − | − | |
AIF | + | ++ | + | − | − | |
Caspase 3 | +++ | +++ | +++ | ++ | + | |
Caspase 8 | + | ++ | − | − | ++ | + |
Caspase 9 | +++ | +++ | +++ | +++ | ++ | ++ |
CDK2 | + | + | − | +++ | +++ | |
CDK4 | − | − | − | − | − | |
CDK6 | + | + | + | +++ | +++ | |
Cyclin D1 | + | + | + | +++ | +++ | |
Cyclin D2 | − | − | − | − | − | − |
Cyclin D3 | ++ | ++ | − | + | + | |
Cyclin E | − | − | − | − | − | |
Transglutaminase I | − | ++ | − | − | − | |
DNA fragmentation factor 45 | +++ | + | + | + | +++ | +++ |
DNase I | +++ | +++ | ++ | + | − | − |
Involucrine | − | ++ | − | ++ | − | − |
p16α | − | − | − | − | − | − |
p16β | − | − | − | − | − | − |