A Large-Scalein SituHybridization System Using an Equalized cDNA Library

doi:10.1006/abio.1997.2399

Analytical Biochemistry

Volume 254, Issue 1, 1 December 1997, Pages 23-30

https://doi.org/10.1006/abio.1997.2399 Get rights and content

Abstract

We have developed a large-scalein situhybridization system in which all the procedures are carried out on a 96-well format: digoxigenin-labeled probes were synthesized from PCR-amplified templates, sections were mounted on 96-well plates, and hybridization and immunohistochemistry protocols were carried out in each well of the plates. This system in combination with equalized (normalized) cDNA library as the source for the probes enables us to identify the cellular distribution of many mRNAs in various tissues rapidly and efficiently. Thus, this system may be a novel cloning method of identify genes differentially expressed in many tissues. In addition, this system has a potential to be automated.

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¹: To whom correspondence should be addressed at Hirohashi Cell Configuration Project, ERATO, Japan Science and Technology Corporation (JST), 5-9-4 Tokodai, Tsukuba, 300-26, Japan. Fax: +81-298-47-5226. E-mail: [email protected].

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Regular ArticleA Large-Scalein SituHybridization System Using an Equalized cDNA Library

Abstract

Genomics

Trends Genet.

Genomics

Cell

Anal. Biochem.

Biochim. Biophys. Acta

J. Biol. Chem.

Gene

J. Biol. Chem.

Anal. Biochem.

Biol. Cell

Anal. Biochem.

Dev. Biol.

Curr. Opin. Genet. Dev.

Nucleic Acids Res.

Proc. Natl. Acad. Sci. USA

Nucleic Acids Res.

Proc. Natl. Acad. Sci. USA

Regular Article
A Large-Scalein SituHybridization System Using an Equalized cDNA Library