Biochemical and Biophysical Research Communications
Regular ArticleDevelopment and Characterization of a New Polyclonal Antibody Specifically against Tissue Inhibitor of Metalloproteinases 4 in Human Breast Cancer☆,☆☆
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Supported in part by grants from the National Institutes of Health (CA78646), the Elsa U. Pardee Foundation, the Gustavus and Louise Pfeiffer Research Foundation (to Q.-X.A.S.), and the Department of Defense Predoctoral Fellowship (DAMD17-00-1-0243, to D.R.H.).
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Abbreviations used: Ab, antibody; ECL, enhanced chemiluminescence; ECM, extracellular matrix; ELISA, enzyme-linked immunosorbent assay; MMP, matrix metalloproteinase; MMP-2, matrix metalloproteinase 2, gelatinase A; MT1-MMP, membrane type 1 matrix metalloproteinase; pAb-T4-S61, a polyclonal antibody against a TIMP-4 peptide starting from residue Ser61; TIMP, tissue inhibitor of metalloproteinases.
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To whom correspondence should be addressed at Department of Chemistry, 203 Dittmer Laboratory of Chemistry Building, Florida State University, Tallahassee, FL 32306-4390. Fax: (850) 644-8281. E-mail: [email protected].