Crystallization Notes
Preliminary Crystallographic Analysis of Bovine Enterovirus

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Abstract

Bovine enterovirus (BEV) strain VG-5-27 derived from an infections cDNA clone has been crystallized as extended hexagonal plates. Virus recovered from crystals produced cytopathic effect in BHK cells. These crystals diffract X-rays from high energy synchrotron sources to beyond 2·7 Å. The crystal system is monoclinic, space group P21 with unit cell dimensions a = 388 Å, b = 390 Å, c = 360 Å, β = 113°. The virion is 300 Ådiameter and one whole particle constitutes the crystallographic asymmetric unit, giving rise to 60-fold non-crystallographic redundancy.

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