Abstract
The ability to target specific sequences of DNA through oligonucleotide-based triple-helix formation provides a powerful tool for genetic manipulation. Under experimental conditions, triplex DNA can inhibit DNA transcription and replication, generate site-specific mutations, cleave DNA, and induce homologous recombination. This review describes the binding requirements for triplex formation, surveys recent advancements in the chemistry and biology of triple helices, and considers several potential applications of triplex DNA for use in genetic therapy.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received: 23 September 1996 / Accepted: 18 October 1996
Rights and permissions
About this article
Cite this article
Chan, P., Glazer, P. Triplex DNA: fundamentals, advances, and potential applications for gene therapy. J Mol Med 75, 267–282 (1997). https://doi.org/10.1007/s001090050112
Issue Date:
DOI: https://doi.org/10.1007/s001090050112