Elsevier

Virology

Volume 78, Issue 2, 1977, Pages 554-566
Virology

Studies on the in vitro uncoating of poliovirus II. Characteristics of the membrane-modified particle

https://doi.org/10.1016/0042-6822(77)90130-1Get rights and content

Abstract

Interaction of type I poliovirus with an extract of isolated HeLa cell membranes resulted in a modification of the viral capsid. As a consequence of the modification, the capsid became sensitive to proteases and detergents, and the sedimentation value of the virion was slightly diminished. Treatment of the modified particle with chymotrypsin reduced its sedimentation value and, although the RNA genome was still encapsidated, it was degradable by RNase. Treatment of the membrane-modified particle with detergent (sodium dodecyl sulfate, SDS) disengaged the viral RNA as an intact (i.e., 35 S) molecule. Each of the three serotypes of poliovirus was modified when treated with membrane extract. They differed, however, in the effect of secondary treatment with chymotrypsin, viz., type I was further modified, but types II and III underwent degradation. When secondary treatment was with SDS, all three released 35 S RNA. The above in vitro reactions of the membrane-modified particle are discussed as possible counterparts of the in vivo uncoating phenomenon.

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Present address: Department of Microbiology and Biochemistry, Idaho State University, Pocatello, Idaho 83209.

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