Cell
Volume 59, Issue 6, 22 December 1989, Pages 1115-1125
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Article
The v-myb oncogene product binds to and activates the promyelocyte-specific mim-1 gene

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Abstract

The v-myb oncogene induces myeloid leukemias in chickens, transforms myeloid cells in vitro, and encodes a sequence-specific DNA binding protein. We used differential hybridization to screen for v-myb-regulated genes in cells transformed by a temperature-sensitive mutant of the oncogene and identified a new gene, mim-1, which encodes a specifically expressed, secretable protein contained in the granules of both normal and v-myb-transformed promyelocytes. The promoter of the mim-1 gene contains three closely spaced binding sites for v-myb protein and is strongly activated by v-myb in a cotransfection assay. Synthetic copies of the binding sites are both necessary and sufficient to confer v-myb protein-dependent activation to a heterologous promoter. We conclude that mim-1 is a cellular gene that is directly regulated by the product of the v-myb oncogene.

References (67)

  • K. Moelling et al.

    DNA-binding activity is associated with purified Myb proteins from AMV and E26 viruses and is temperature-sensitive for E26 ts mutants

    Cell

    (1985)
  • S.A. Ness et al.

    Carbamoyl-phosphate synthetases from Neurospora crassa: immunological relatedness of the enzymes from Neurospora, bacteria, yeast and mammals

    J. Biol. Chem.

    (1985)
  • S.A. Ness et al.

    v-myb dominance over v-myc in doubly transformed chick myelomonocytic cells

    Cell

    (1987)
  • J.B. Olmsted

    Affinity purification of antibodies from diazotized paper blots of heterogeneous protein samples

    J. Biol. Chem.

    (1981)
  • K. Radke et al.

    Transformation of both erythroid and myeloid cells by E26, an avian leukemia virus that contains the myb gene

    Cell

    (1982)
  • B. Vennström et al.

    Isolation and characterization of chicken DNA homologous to the two putative oncogenes of avian erythroblastosis virus

    Cell

    (1982)
  • K. Weston et al.

    Transcriptional activation by the v-myb oncogene and its cellular progenitor, c-myb

    Cell

    (1989)
  • J.M. Almendral et al.

    Complexity of the early genetic response to growth factors in mouse fibroblasts

    Mol. Cell. Biol.

    (1988)
  • H. Bading et al.

    Selective DNA binding of the human cellular myb protein isolated by immunoaffinity chromatography using a monoclonal antibody

    Oncogene

    (1987)
  • J.A. Bartlett et al.

    Sequencing of supercoiled plasmid DNA

    Biotechniques

    (1986)
  • H. Beug et al.

    Reversibility of differentiation and proliferative capacity in avian myelomonocytic cells transformed by tsE26 leukemia virus

    Genes Dev.

    (1987)
  • H. Biedenkapp et al.

    Viral myb oncogene encodes a sequence-specific DNA-binding activity

    Nature

    (1988)
  • D. Bohmann et al.

    A transcription factor which binds to the enhancers of SV40, immunoglobulin heavy chain and U2 snRNA genes

    Nature

    (1987)
  • C. Bonnerot et al.

    A β-galactosidase hybrid protein targeted to nuclei as a marker for developmental studies

  • W.J. Boyle et al.

    Avian myeloblastosis virus and E26 virus oncogene products are nuclear proteins

  • B. Burke et al.

    A monoclonal antibody against a 135-K Golgi membrane protein

    EMBO J.

    (1982)
  • S.A. Chuvpilo et al.

    A simple and rapid method for sequencing DNA

    FEBS Lett.

    (1984)
  • M.F. Clarke et al.

    Constitutive expression of a c-myb cDNA blocks Friend murine erythroleukemia cell differentiation

    Mol. Cell. Biol.

    (1988)
  • R.W. Craig et al.

    Early decline in c-myb oncogene expression in the differentiation of human myeloblastic leukemia (ML-1) cells induced with 12-O-tetradecanoylphorbol-13-acetate

    Cancer Res.

    (1984)
  • J.R. de Wet et al.

    Firefly luciferase gene: structure and expression in mammalian cells

    Mol. Cell. Biol.

    (1987)
  • C. Desplan et al.

    The Drosophila developmental gene, engrailed, encodes a sequence-specific DNA binding activity

    Nature

    (1985)
  • J. Devereux et al.

    A comprehensive set of sequence analysis programs for the VAX

    Nucl. Acids Res.

    (1984)
  • S.P. Duprey et al.

    Developmental regulation of c-myb in normal myeloid progenitor cells

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