Cell
Volume 73, Issue 7, 2 July 1993, Pages 1423-1434
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Fibrinogen mediates leukocyte adhesion to vascular endothelium through an ICAM-1-dependent pathway

https://doi.org/10.1016/0092-8674(93)90367-YGet rights and content

Abstract

Leukocyte traffic in immune-inflammatory responses requires regulated adhesion of leukocyte subsets to vascular endothelium. We show that fibrinogen or normal human plasma enhances by 2- to 5-fold the adhesion of cells of myeloid and lymphoid lineage to endothelium. This mechanism is mediated by fibrinogen binding to complementary membrane receptors on leukocytes and endothelial cells. Using an affinity chromatography purification strategy, genetically engineered transfectants, and direct binding studies to the isolated recombinant protein, we identified a novel hematopoietic fibrinogen receptor participating in this adhesion pathway as intercellular adhesion molecule 1 (ICAM-1). Accordingly, a new model can be proposed, in which fibrinogen binding to a variety of vascular cell receptors mediates a specific pathway of cell to cell adhesion by bridging together leukocytes and endothelial cells.

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  • Cited by (0)

    Permanent address: DBMS/Laboratory of Hematology, Unité217, Institut National de la Santéet de la Recherche Médicale, F38041 Grenoble Cedex, France.

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    Present address: Center for Thrombosis and Vascular Biology, The Cleveland Clinic Foundation, Cleveland, Ohio 44195.

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