Alimentary TractPrognostic significance of allelic loss at chromosome 18q21 for stage II colorectal cancer☆,☆☆
Section snippets
Patient selection and data collection
Seventy patients with stage II colorectal cancer (Dukes' B2 lesions; invasive through the muscularis propria but lymph node negative) for whom pathology slides were available were identified consecutively from the tumor registry at Ohio State University from 1984 to 1988 and the tumor registry at the University of Michigan from 1984 to 1989. This period was chosen because postoperative adjuvant chemotherapy was not administered routinely to patients commonly at these institutions until after
Results
We analyzed 70 stage II colorectal tumors in a blinded fashion for allelic loss at five 18q21 DNA microsatellite markers that are tightly linked to the DCC gene locus, including an intragenic microsatellite located within the DCC gene (18q DCC-TA) (Figure 1).
Discussion
The purpose of this study was to validate in a blinded fashion whether allelic loss at chromosome 18q21 around and within the DCC gene is a significant prognostic indicator of survival in stage II colorectal cancer. In our patient population, we were unable to find an association between 18q21 allelic loss and survival by univariate analysis and multivariate analysis.
Our conclusions differ greatly from those of Jen et al.7 Comparison with their study shows a similar number of stage II patients
Acknowledgements
The authors thank Sajeev Cherian for assistance.
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Cited by (0)
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Supported by National Institutes of Health grants DK02433, CA72851, and CA57716; the Robert Wood Johnson Foundation; the “V” Foundation for Cancer Research; and the Veterans Administration Research Service.
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Address requests for reprints to: John M. Carethers, M.D., Department of Medicine 0688, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093-0688. e-mail: [email protected]; fax: (619) 822-0301.