Engineering antibodies for imaging and therapy
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Cited by (70)
Monoclonal antibody-based optical molecular imaging probes; considerations and caveats in chemistry, biology and pharmacology
2016, Current Opinion in Chemical BiologyCitation Excerpt :Interestingly, the HAMA response is less limiting in immunocompromised patients such as those with B-cell lymphoma or hematologic malignancies. This problem was largely eliminated by creating chimeric or humanized antibodies, which combine the variable region or the complimentary determining regions (CDRs), respectively, of murine antibodies with the constant regions of human antibodies [2]. Finally, large phage display libraries or humanized mice can be used to produce fully human antibodies, though the latter method permits only a limited variety of antibody production (Figure 1).
Optimal fusion of antibody binding domains resulted in higher affinity and wider specificity
2015, Journal of Bioscience and BioengineeringGlioblastoma cancer stem cells: Biomarker and therapeutic advances
2014, Neurochemistry InternationalCitation Excerpt :The result is a polyclonal pool, which is then screened and sequenced for individual clones. Unique clones can then be engineered into immunoglobulin formats to test for therapeutic efficacy (Roovers et al., 2001; Carter and Merchant, 1997). Two recent studies have explored phage display technology against GSCs.
Iron oxide nanoparticles for targeted cancer imaging and diagnostics
2012, Nanomedicine: Nanotechnology, Biology, and MedicineAntibody Fab display system that can perform open-sandwich ELISA
2009, Analytical BiochemistryCitation Excerpt :Using a nonsuppressing host HB2151, we could actually produce a soluble Fab fragment in the culture supernatant, which enables rapid production and evaluation of chimeric Fab protein. Nowadays humanized and human antibodies are of particular interest, since they are considered to be valuable for diagnostic and therapeutic applications [21], avoiding the HAMA (human anti-mouse antibody) response frequently observed with rodent antibodies. OS-ELISA with a full-length Fab fragment with the C-terminal cysteine of the kappa chain on phage was not very successful.
A broad range of Fab stabilities within a host of therapeutic IgGs
2007, Biochemical and Biophysical Research Communications