Abstract
The centrosomes are thought to maintain genomic stability through the establishment of bipolar spindles during cell division, ensuring equal segregation of replicated chromosomes to two daughter cells. Deregulated duplication and distribution of centrosomes have been implicated in chromosome segregation abnormalities, leading to aneuploidy seen in many cancer cell types. Here, we report that STK15 (also known as BTAK and aurora2 ), encoding a centrosome-associated kinase, is amplified and overexpressed in multiple human tumour cell types, and is involved in the induction of centrosome duplication-distribution abnormalities and aneuploidy in mammalian cells. STK15 amplification has been previously detected in breast tumour cell lines 1 and in colon tumours 2 ; here, we report its amplification in approximately 12% of primary breast tumours, as well as in breast, ovarian, colon, prostate, neuroblastoma and cervical cancer cell lines. Additionally, high expression of STK15 mRNA was detected in tumour cell lines without evidence of gene amplification. Ectopic expression of STK15 in mouse NIH 3T3 cells led to the appearance of abnormal centrosome number (amplification) and transformation in vitro . Finally, overexpression of STK15 in near diploid human breast epithelial cells revealed similar centrosome abnormality, as well as induction of aneuploidy. These findings suggest that STK15 is a critical kinase-encoding gene, whose overexpression leads to centrosome amplification, chromosomal instability and transformation in mammalian cells.
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Acknowledgements
Authors are grateful to R. Balczon for supplying the auto-antibodies against the centrosome and to R.E. Palazzo for the two rabbit antisera against γ tubulin. Technical assistance of M. Nelman Gonzalez, S. Che, A. Wagh, R. Nandagiri and the help of M. Pearlman in counting the soft agar colonies and S. Zhao in nucleotide sequence analyses are acknowledged. We thank I. Hernandez, L. Benson and D. Sprabary for help in preparing this manuscript and the Department of Scientific Publications at UTMDACC for editorial suggestions. This work was supported in part by grants from the Physicians Referral Service, Breast Cancer Basic Research Program of the University of Texas M.D. Anderson Cancer Center, from NIH (CA 61979) to S.S. and (CA 41424, CA 64255) to B.R.B. The M.D. Anderson DNA sequencing facility is supported by the NIH core grant CA 16672.
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Zhou, H., Kuang, J., Zhong, L. et al. Tumour amplified kinase STK15/BTAK induces centrosome amplification, aneuploidy and transformation. Nat Genet 20, 189–193 (1998). https://doi.org/10.1038/2496
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DOI: https://doi.org/10.1038/2496
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