Abstract
THE cytoplasmic proteins β-catenin of vertebrates and armadillo of Drosophila have two functions: they link the cadherin cell-adhesion molecules to the cytoskeleton1–4, and they participate in the wnt/wingless signalling pathway5–7. Here we show, in a yeast two-hybrid screen, that the architectural transcription factor LEF-1 (for lymphoid enhancer-binding factor)8–10 interacts with β-catenin. In mammalian cells, coexpressed LEF-1 and β-catenin form a complex that is localized to the nucleus and can be detected by immunoprecipitation. Moreover, LEF-1 and β-catenin form a ternary complex with DNA that displays an altered DNA bend. Microinjection of LEF-1 into Xenopus embryos induces axis duplication, which is augmented by interaction with β-catenin. Thus β-catenin regulates gene expression by direct interaction with transcription factors such as LEF-1, providing a molecular mechanism for the transmission of signals from cell-adhesion components or wnt protein to the nucleus.
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References
Hülsken, J., Birchmeier, W. & Behrens, J. J. Cell Biol. 127, 2061–2069 (1994).
Aberle, H. et al. J. Cell Sci. 107, 3655–3663 (1994).
Nagafuchi, A., Ishihara, S. & Tsukita, A. J. Cell Biol. 127, 235–245 (1994).
McCrea, P. D., Turck, C. W. & Gumbiner, B. Science 254, 1359–1361 (1991).
Peifer, M., Berg, S. & Reynolds, A. B. Cell 76, 789–791 (1994).
Noordermeer, J., Klingensmith, J., Perrimon, N. & Nusse, R. Nature 367, 80–83 (1994).
Funayama, N., Fagotto, F., McCrea, P. & Gumbiner, B. J. Cell Biol. 128, 959–968 (1995).
Travis, A., Amsterdam, A., Belanger, C. & Grosschedl, R. Genes Dev. 5, 880–894 (1991).
Waterman, M. L., Fischer, W. H. & Jones, K. Genes Dev. 5, 656–669 (1991).
Giese, K., Kingsley, C., Kirschner, J. R. & Grosschedl, R. Genes Dev. 9, 995–1008 (1995).
Fields, S. & Song, O. K. Nature 340, 245–246 (1989).
Prieve, M. G., Guttridge, K. L., Munguia, J. E. & Waterman, M. L. J. Biol. Chem. 271, 7654–7658 (1996).
Cowin, P., Kapprell, J.-P., Franke, W. W., Tamkun, J. & Hynes, R. O. Cell 46, 1063–1073 (1986).
Giese, K., Cox, J. & Grosschedl, R. Cell 69, 185–195 (1992).
Love, J. J. et al. Nature 376, 791–795 (1995).
Kerppola, T. K. & Curran, T. Cell 66, 317–326 (1991).
Kratochwil, K., Dull, M., Farinas, I., Galceran, J. & Grosschedl, R. Genes Dev. 10, 1382–1394 (1996).
McMahon, A. P. & Moon, R. T. Cell 58, 1075–1084 (1989).
Sokol, S. Y., Klingensmith, J., Perrimon, N. & Itoh, K. Development 121, 1637–1647 (1995).
He, X., Saint-Jeannet, J.-P., Woodgett, J. R., Varmus, H. E. & Dawid, I. Science 374, 617–622 (1995).
Heasman, J. et al. Cell 79, 791–803 (1994).
Peifer, M., Sweeton, D., Casey, M. & Wieschaus, E. Development 120, 369–380 (1994).
Bhanot, P. et al. Nature 382, 225–230 (1996).
Munemitsu, S., Albert, I., Souza, B., Rubinfeld, B. & Polakis, P. Proc. Natl Acad. Sci. USA 92, 3046–3050 (1995).
Miller, J. H. Experiments in Molecular Genetics (Cold Spring Harbor Laboratory Press, NY, 1972).
Giese, K. & Grosschedl, R. EMBO J. 12, 4667–4676 (1993).
Carlson, P., Waterman, M. L. & Jones, K. A. Genes Dev. 7, 2418–2430 (1993).
Kim, J., Zwieb, C., Wu, C. & Adhya, S. Gene 85, 15–23 (1989).
Krieg, P. & Melton, D. Nucleic Acids Res. 12, 7057–7070 (1984).
Kühl, M., Finnemann, S., Binder, O. & Wedlich, D. Mech. Dev. 54, 71–82 (1996).
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Behrens, J., von Kries, J., Kühl, M. et al. Functional interaction of β-catenin with the transcription factor LEF-1. Nature 382, 638–642 (1996). https://doi.org/10.1038/382638a0
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DOI: https://doi.org/10.1038/382638a0
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