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Co-operation of Simian virus 40 T antigen and insulin receptor substrate-1 in protection from apoptosis induced by Interleukin-3 withdrawal

Abstract

32D cells are interleukin-3 (IL-3) dependent murine hemopoietic cells, that undergo apoptosis after IL-3 withdrawal. An overexpressed insulin-like growth factor I receptor (IGF-IR) protects these cells from apoptosis induced by IL-3 withdrawal. When 32D cells are stably transfected with plasmids expressing either IRS-1 (a major substrate of the IGF-IR) or the Simian virus 40 large T antigen, singly, they still undergo apoptosis after IL-3 withdrawal, although IRS-1 offers partial protection. The cells, however, are fully protected when they are stably transfected with both IRS-1 and SV40 T antigen. Protection from apoptosis in these cells is characterized by the stabilization of the Stat1 and Stat5 protein levels, whose synthesis is inhibited when IL-3 is withdrawn.

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Zhou-Li, F., Xu, SQ., Dews, M. et al. Co-operation of Simian virus 40 T antigen and insulin receptor substrate-1 in protection from apoptosis induced by Interleukin-3 withdrawal. Oncogene 15, 961–970 (1997). https://doi.org/10.1038/sj.onc.1201265

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  • DOI: https://doi.org/10.1038/sj.onc.1201265

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