Alimentary TractDistinct chromosomal aberrations in Epstein-Barr virus–carrying gastric carcinomas tested by comparative genomic hybridization☆
Section snippets
Clinical specimens
The identification of EBV-carrying gastric carcinomas was performed by EBER1/2-RNA in situ hybridization as described previously.6
Of 132 gastric adenocarcinomas, collected at the Department of Pathology of the University Hospital Vrije Universiteit, Amsterdam, the Netherlands, including 10 EBV-carrying gastric carcinomas, as has been published recently,6 CGH data had been obtained in 44 cases.15 None of the EBV-carrying gastric carcinomas used in this study were of the lymphoepithelioma-like
Chromosomal aberrations in EBV-carrying and EBV-negative gastric adenocarcinomas
The mean number of chromosomal losses in EBV-carrying gastric carcinomas was clearly higher than in EBV-negative gastric carcinomas (9.4 [range, 0–14] and 6.7 [range, 0–15] respectively) but this difference did not reach statistical significance. The mean of chromosomal events was also higher in EBV-carrying gastric carcinomas (12 vs. 10, not significant). In contrast, the mean number of chromosomal gains was lower in the EBV-carrying gastric carcinomas (2.6 vs. 3.3 [range, 0–13 each], not
Discussion
The present study is the first study to investigate genetic instability on the chromosomal level comparing EBV-carrying and EBV-negative gastric carcinomas using CGH analysis. Genetic instability on the chromosomal level is the major type of genetic instability in gastric cancer. The chromosomal changes detected by CGH analysis might point at specific chromosomal regions that might play a role in the pathogenesis of these carcinomas. We find chromosomal aberrations which are either restricted
References (28)
- et al.
Epstein-Barr virus-associated gastric carcinomas: relation to H. pylori infection and genetic alterations
Gastroenterology
(2000) - et al.
Loss of p16/CDKN2A tumor suppressor protein in gastric adenocarcinoma is associated with Epstein-Barr virus and anatomic location in the body of the stomach
Hum Pathol
(2000) - et al.
Comparative genomic hybridization analysis of natural killer cell lymphoma/leukemia. Recognition of consistent patterns of genetic alterations
Am J Pathol
(1999) - et al.
Genetic alterations of gastric cancer: comparative genomic hybridization and fluorescence in situ hybridization studies
Cancer Genet Cytogenet
(2000) - et al.
Loss of heterozygosity on the short arm of chromosome 3 in nasopharyngeal carcinoma
Cancer Genet Cytogenet
(1991) - et al.
The role of the Epstein-Barr virus in the oncogenesis of EBV(+) gastric carcinomas
Virchows Arch
(1999) - et al.
Epstein-Barr virus and carcinomas: rare association of the virus with gastric adenocarcinomas
Br J Cancer
(1993) - et al.
Epstein-Barr virus-associated gastric adenocarcinoma
Am J Pathol
(1992) - et al.
Helicobacter pylori and Epstein-Barr virus infection and the p53 tumour-suppressor pathway in gastric stump cancer compared with carcinoma in the non-operated stomach
J Clin Pathol
(1998) - et al.
Epstein-Barr virus in gastric carcinoma with lymphoid stroma. Special reference to its detection by the polymerase chain reaction and in situ hybridization in 99 tumors, including a morphologic analysis
Cancer
(1994)
Unique transcription pattern of Epstein-Barr virus in EBV-carrying gastric adenocarcinomas: expression of the transforming BARF1 gene
Cancer Res
Microsatellite instability in the progression of gastric carcinoma
Cancer Res
Microsatellite instability, Epstein-Barr virus, mutation of type II transforming growth factor beta receptor and BAX in gastric carcinomas in Hong Kong Chinese
Br J Cancer
Prognostic value and clinicopathological profile of microsatellite instability in gastric cancer
Clin Cancer Res
Cited by (45)
Epithelial Neoplasms of the Stomach
2009, Surgical Pathology of the GI Tract, Liver, Biliary Tract and PancreasEpithelial Neoplasms of the Stomach
2008, Surgical Pathology of the GI Tract, Liver, Biliary Tract and Pancreas: Expert Consult - Online and PrintEBV-Positive Gastric Cancer: Current Knowledge and Future Perspectives
2020, Frontiers in OncologyProgress of EB virus-positive gastric cancer
2020, Cancer Research and ClinicEpstein-Barr virus particles induce centrosome amplification and chromosomal instability
2017, Nature Communications
- ☆
Address requests for reprints to: Adriaan van den Brule, Ph.D., Department of Pathology, Molecular Pathology Section, University Hospital Vrije Universiteit, P.O. Box 7057, 1007 MB, Amsterdam, The Netherlands. e-mail: [email protected]; fax: (31) 20 444 2964.