Abstract
Latent membrane protein 1 (LMP1) is an Epstein-Barr virus (EBV) protein expressed in EBV-transformed B lymphocytes and in approximately 50% of nasopharyngeal carcinomas (NPCs). LMP1 signaling involves several cellular signaling intermediates, especially TNF receptor-associated factors (TRAFs). We have shown previously that LMP1 is highly concentrated in a cell fraction called glycosphingolipid-rich membrane complexes (GSL complexes). We report here that parallel accumulation of LMP1 and TRAF3, but not TRAF1 or TRADD, was observed in GSL complexes from lymphoblastoid and LMP1-positive NPC cells. In contrast, TRAF3 was not concentrated in GSL complexes from LMP1-negative cells. Binding of LMP1 and TRAF3 in GSL complexes was demonstrated in lymphoblastoid and NPC cells, by co-immunoprecipitation with both anti-LMP1 and anti-TRAF3 antibodies.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Biopsy
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Burkitt Lymphoma / chemistry
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Burkitt Lymphoma / metabolism*
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Burkitt Lymphoma / pathology
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Centrifugation, Density Gradient
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Glycosphingolipids / isolation & purification
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Glycosphingolipids / metabolism*
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Herpesvirus 4, Human / genetics
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Humans
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Mice
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Mice, Nude
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Nasopharyngeal Neoplasms / chemistry
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Nasopharyngeal Neoplasms / metabolism*
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Nasopharyngeal Neoplasms / pathology
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Proteins / genetics
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Proteins / isolation & purification
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Proteins / metabolism*
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Receptors, Tumor Necrosis Factor / physiology
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TNF Receptor-Associated Factor 3
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Transplantation, Heterologous
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Tumor Cells, Cultured
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Viral Matrix Proteins / genetics
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Viral Matrix Proteins / isolation & purification
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Viral Matrix Proteins / metabolism*
Substances
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EBV-associated membrane antigen, Epstein-Barr virus
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Glycosphingolipids
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Proteins
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Receptors, Tumor Necrosis Factor
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TNF Receptor-Associated Factor 3
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Viral Matrix Proteins