Abstract
A growth assay using the fluorescent dye Hoechst 33258 has been developed which allows sensitive and rapid analysis of the DNA content of a variety of cell types including keratinocytes and mucus-secreting cells, and which requires a minimum of liquid handling. The assay can detect as few as 500 diploid human cells, and is compatible with the simultaneous detection of [3H]thymidine incorporation in the same cultures.
MeSH terms
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Animals
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Breast Neoplasms / genetics
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Breast Neoplasms / metabolism
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Breast Neoplasms / pathology
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Cattle
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Cell Division / physiology
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Cells, Cultured
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DNA / analysis*
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DNA, Neoplasm / analysis
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Fluorometry / methods
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Humans
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Keratinocytes / cytology
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Keratinocytes / metabolism
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Pancreatic Neoplasms / genetics
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Pancreatic Neoplasms / metabolism
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Pancreatic Neoplasms / pathology
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Sensitivity and Specificity
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Solvents
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Thymidine / metabolism
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Tritium
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Tumor Cells, Cultured
Substances
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DNA, Neoplasm
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Solvents
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Tritium
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DNA
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Thymidine