CSF pretreatment and the diagnosis of herpes encephalitis using the polymerase chain reaction

C Dennett; P E Klapper; G M Cleator; A G Lewis

doi:10.1016/0166-0934(91)90125-j

CSF pretreatment and the diagnosis of herpes encephalitis using the polymerase chain reaction

J Virol Methods. 1991 Sep;34(1):101-4. doi: 10.1016/0166-0934(91)90125-j.

Authors

C Dennett¹, P E Klapper, G M Cleator, A G Lewis

Affiliation

¹ Department of Pathological Sciences, University of Manchester, U.K.

PMID: 1659579
DOI: 10.1016/0166-0934(91)90125-j

Abstract

A number of techniques for extraction of DNA prior to polymerase chain reaction (PCR) amplification of herpes simplex virus (HSV) DNA in cerebrospinal fluid (CSF) were compared to the use of "native" CSF in the PCR reaction. The results indicate that extraction of DNA (which allows efficient removal of inhibitors of Taq polymerase) is an essential pre-requisite of the PCR detection of CSF HSV DNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA, Viral / cerebrospinal fluid*
Encephalitis / diagnosis*
Encephalitis / microbiology
Herpes Simplex / cerebrospinal fluid
Herpes Simplex / diagnosis*
Humans
Polymerase Chain Reaction / methods*
Simplexvirus / genetics*

Substances

DNA, Viral