Abstract
GAL80, an inhibitor of the yeast transcriptional activator GAL4, is converted into an activator by inserting an acidic activating sequence into it. This hybrid activator does not bind to DNA directly, but is brought to DNA by interacting with a derivative of GAL4 that interacts with both DNA and GAL80.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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DNA, Fungal / metabolism
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Galactose / metabolism*
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Gene Expression Regulation*
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Genes, Fungal
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Transcription Factors / genetics*
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Transcription Factors / metabolism
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Transcription, Genetic*
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Yeasts / genetics*
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Yeasts / metabolism
Substances
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DNA, Fungal
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Recombinant Fusion Proteins
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Transcription Factors
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Galactose