Abstract
The complete 1,210-amino acid sequence of the human epidermal growth factor (EGF) receptor precursor, deduced from cDNA clones derived from placental and A431 carcinoma cells, reveals close similarity between the entire predicted v-erb-B mRNA oncogene product and the receptor transmembrane and cytoplasmic domains. A single transmembrane region of 23 amino acids separates the extracellular EGF binding and cytoplasmic domains. The receptor gene is amplified and apparently rearranged in A431 cells, generating a truncated 2.8-kilobase mRNA which encodes only the extracellular EGF binding domain.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Carcinoma, Squamous Cell
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Cell Line
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DNA / metabolism*
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Epidermal Growth Factor / metabolism
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ErbB Receptors
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Gene Amplification*
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Genes*
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Humans
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Nucleic Acid Hybridization
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Peptide Fragments / analysis
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RNA, Messenger / genetics
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Receptors, Cell Surface / genetics*
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Receptors, Cell Surface / metabolism
Substances
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Peptide Fragments
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RNA, Messenger
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Receptors, Cell Surface
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Epidermal Growth Factor
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DNA
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ErbB Receptors
Associated data
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GENBANK/K01885
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GENBANK/K02047
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GENBANK/X00588
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GENBANK/X00663