Leukaemia inhibitory factor (LIF) production in a mouse model of spinal trauma

Neurosci Lett. 1998 Jun 12;249(1):1-4. doi: 10.1016/s0304-3940(98)00360-7.

Abstract

A model of spinal trauma was developed where spinal neurones of adult mice were exposed to the excitotoxic glutamate analogue beta-N-oxylamino-L-alanine (L-BOAA). After 24 h, the injured neurones received a single dose of [125I]-LIF at the same site of the spinal cord, and 2 h later, tissues were removed to assess the distribution of leukaemia inhibitory factor (LIF). There was a significant increase in LIF binding to the injured region of the spinal cord over saline controls, and this corresponded with a significant increase in LIF mRNA expression in the same region of the cord. There was a change in the expression of ciliary neurotrophic factor, but the expression of cardiotrophin-1 (CT-1) and the common receptor subunit LIF receptor beta (LIFRbeta) did not change after neurotoxin treatment. The results add to the evidence that LIF plays a significant role in the response of adult neuronal tissue to injury.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids, Diamino*
  • Animals
  • Ciliary Neurotrophic Factor
  • Cytokines / biosynthesis
  • Growth Inhibitors / biosynthesis*
  • Growth Inhibitors / metabolism
  • Interleukin-6*
  • Leukemia Inhibitory Factor
  • Leukemia Inhibitory Factor Receptor alpha Subunit
  • Lymphokines / biosynthesis*
  • Lymphokines / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Motor Neuron Disease / chemically induced
  • Motor Neuron Disease / metabolism*
  • Motor Neuron Disease / pathology
  • Motor Neurons / metabolism
  • Nerve Tissue Proteins / biosynthesis
  • Neurotoxins / toxicity
  • RNA, Messenger / biosynthesis
  • Receptors, Cytokine / biosynthesis
  • Receptors, Cytokine / metabolism
  • Receptors, OSM-LIF
  • Spinal Cord / metabolism
  • Spinal Cord / pathology
  • Spinal Diseases / chemically induced
  • Spinal Diseases / metabolism*
  • Spinal Diseases / pathology
  • Up-Regulation
  • beta-Alanine / analogs & derivatives
  • beta-Alanine / toxicity

Substances

  • Amino Acids, Diamino
  • Ciliary Neurotrophic Factor
  • Cytokines
  • Growth Inhibitors
  • Interleukin-6
  • Leukemia Inhibitory Factor
  • Leukemia Inhibitory Factor Receptor alpha Subunit
  • Lif protein, mouse
  • Lifr protein, mouse
  • Lymphokines
  • Nerve Tissue Proteins
  • Neurotoxins
  • RNA, Messenger
  • Receptors, Cytokine
  • Receptors, OSM-LIF
  • beta-Alanine
  • oxalyldiaminopropionic acid
  • cardiotrophin 1