Article Text

Download PDFPDF
Rapid differential diagnosis of myxoid liposarcoma by fluorescence in situ hybridisation on cytological preparations
  1. A Mezzelani,
  2. G Sozzi,
  3. M A Pierotti,
  4. S Pilotti
  1. Divisione di Anatomia Patologica e Citologia, Istituto Nazionale dei Tumori, Milan, Italy
  2. Divisione di Oncologia Sperimentale A


    In two cases of suspected myxoid liposarcoma, where chromosomal metaphase preparations were not available, fluorescence in situ hybridisation was performed on interphase nuclei of cytological preparations for the detection of the specific translocation, t(12;16), characteristic of this tumour and of trisomy 8, which is the most frequent secondary chromosome aberration. Probes directed against chromosomes 12 and 16 and against the centromeres of chromosomes 12 and 8 were hybridised on cell brushings and cytocentrifuge preparations. The finding of three painting domains of both chromosomes 12 and 16 and of only two signals with the centromeric probe directed against chromosome 12, suggested the presence of t(12;16) in both cases. In one case trisomy 8 was inferred from the occurrence of three centromere 8 signals. This approach can be used to detect specific chromosomal abnormalities when an urgent differential diagnosis is requested or when chromosome preparations are not available, or both.

    • fluorescence in situ hybridisation
    • chromosomal metaphase preparations
    • myxoid liposarcoma

    Statistics from

    Request Permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.