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Housekeeping genes confound mRNA assays in asthma

https://doi.org/10.1136/mp.56.1.59

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    A n Australian study is the first to question routine use in asthma research of two genes widely used for standardising quantitative mRNA assays. The expression of β actin and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) needs careful validation first, say the researchers.

    They used a reverse transcriptase (RT)-PCR method that does not …

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