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Increased zinc finger protein zFOC1 transcripts in gastric cancer compared with normal gastric tissue
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  1. R L Stephen1,
  2. J E Crabtree1,
  3. T Yoshimura1,
  4. C L Clayton2,
  5. M F Dixon1,
  6. P A Robinson1
  1. 1Molecular Medicine Unit, St James’s University Hospital, Leeds LS9 7TF, West Yorkshire, UK
  2. 2Genomics Unit, Glaxo Smith Kline Research and Development, Stevenage SG1 2NY, Hertfordshire, UK
  1. Correspondence to:
 Dr J E Crabtree, Level 7, Clinical Sciences Building, St James’s University Hospital, Leeds LS9 7TF, UK;
 msjjc{at}stjames.leeds.ac.uk

Abstract

Background: Screening of cDNA arrays of the IMAGE library identified human zFOC1 as a differentially expressed cDNA that was upregulated in KATO III gastric cancer cells following stimulation with the gastric pathogen Helicobacter pylori.

Aims: To determine the expression of zFOC1 in gastric mucosa with and without H pylori infection and in patients with gastric cancer.

Results: zFOC1 is localised on chromosome 12q24.3 and encodes a zinc finger protein. Expression studies in human H pylori infected and uninfected gastric biopsies, gastric tumours, and gastric cancer cell lines revealed that zFOCI gene transcripts are significantly higher in gastric cancer than in non-cancerous gastric tissues.

Conclusions: The zFOC1 gene appears to be a tumour marker associated with gastric cancer.

  • gastric cancer
  • zFOC1
  • zinc finger protein
  • cDNA array
  • Helicobacter pylori
  • EST, expressed sequence tag
  • GAPDH, glyceraldehyde 3 phosphate dehydrogenase
  • PAI, pathogenicity island
  • RT-PCR, reverse transcription polymerase chain reaction
  • SDS, sodium dodecyl sulfate
  • SCC, saline sodium citrate
  • UTR, untranslated region
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