Background: Screening of cDNA arrays of the IMAGE library identified human zFOC1 as a differentially expressed cDNA that was upregulated in KATO III gastric cancer cells following stimulation with the gastric pathogen Helicobacter pylori.
Aims: To determine the expression of zFOC1 in gastric mucosa with and without H pylori infection and in patients with gastric cancer.
Results: zFOC1 is localised on chromosome 12q24.3 and encodes a zinc finger protein. Expression studies in human H pylori infected and uninfected gastric biopsies, gastric tumours, and gastric cancer cell lines revealed that zFOCI gene transcripts are significantly higher in gastric cancer than in non-cancerous gastric tissues.
Conclusions: The zFOC1 gene appears to be a tumour marker associated with gastric cancer.
- gastric cancer
- zinc finger protein
- cDNA array
- Helicobacter pylori
- EST, expressed sequence tag
- GAPDH, glyceraldehyde 3 phosphate dehydrogenase
- PAI, pathogenicity island
- RT-PCR, reverse transcription polymerase chain reaction
- SDS, sodium dodecyl sulfate
- SCC, saline sodium citrate
- UTR, untranslated region
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