Figure 2 The transforming growth factor ß (TGFß) responsive enhancer
(TGFßRE) of the ccn2 (ctgf) promoter is required for the increased basal
ccn2 (ctgf) promoter activity seen in scleroderma fibroblasts. The figure is
adapted from data in Holmes et al.30 Three constructs were transfected
into normal dermal and scleroderma dermal fibroblasts: a wild-type
(nucleotides -805 to +17) ccn2 (ctgf) promoter�SEAP reporter construct
(FL), and otherwise identical constructs that contained a mutated TGFßRE
element (TGFßRE) or SMAD element (SMAD).30 Values shown are
relative to FL construct expression in normal fibroblasts. The standard error
in all cases was less than 25%. Mutating the TGFßRE reduces basal ccn2
(ctgf) promoter activity in normal and scleroderma fibroblasts, whereas
mutating the SMAD element has no effect on basal expression.

Corrected Figure 2