TY - JOUR T1 - A novel polymerase chain reaction assay to detect <em>Mycoplasma genitalium</em> JF - Molecular Pathology JO - Mol Pathol SP - 25 LP - 28 DO - 10.1136/mp.56.1.25 VL - 56 IS - 1 AU - K Eastick AU - J P Leeming AU - E O Caul AU - P J Horner AU - M R Millar Y1 - 2003/02/01 UR - http://mp.bmj.com/content/56/1/25.abstract N2 - Aims: To design and validate a polymerase chain reaction (PCR) assay targeting the 16S rRNA gene of Mycoplasma genitalium. Methods: Primers were designed that were complementary to the 16S rRNA gene sequence of M genitalium. After optimisation of the reaction conditions, the PCR was tested against nine M genitalium strains, a dilution series of M genitalium DNA, and a panel of common microorganisms. The PCR was also challenged in parallel with a published assay against 54 urine specimens from men with urethritis. Results: The expected 341 bp product was produced on amplification of material from all M genitalium strains and from none of the other microorganisms tested. The lower limit of detection was 50 genome copies. The new assay detected M genitalium DNA in nine of 54 men with urethritis, in comparison with eight positive specimens detected with the alternative PCR. Conclusions: This novel PCR targeting the M genitalium 16S rRNA gene has been optimised and now provides a sensitive and specific alternative or addition to the available MgPa gene targeting assays. ER -