RT Journal Article
SR Electronic
T1 Tempero–spatial dissociation between the expression of Fas and apoptosis after coronary occlusion
JF Molecular Pathology
JO Mol Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 362
OP 367
DO 10.1136/mp.56.6.362
VO 56
IS 6
A1 Q Z Feng
A1 T D Li
A1 L X Wei
A1 X Qiao
A1 J Yi
A1 L Wang
A1 T S Yang
YR 2003
UL http://mp.bmj.com/content/56/6/362.abstract
AB Aims: To explore the role of Fas in cardiomyocytic apoptosis induced by ischaemia through determining the histological relation between Fas expression and apoptosis in rat myocardium during ischaemia/infarction. Methods: The myocardial ischaemia model was produced by ligating the left coronary artery in Sprague-Dawley rats. The rats were killed from 10 minutes to seven days after surgery. Apoptotic myocardial cells were detected by the in situ terminal deoxynucleotidyl transferase mediated nick end labelling method, and the expression of Fas by immunohistochemistry and western blotting. Results: Cardiomyocytic apoptosis appeared from three to 36 hours after ischaemia. The expression of Fas could be detected by western blot from before surgery to seven days of ischaemia. Apoptosis and the expression of Fas in the cardiomyocytes appeared in different regions of the myocardium: apoptosis in the ischaemic region, Fas in the regions surrounding ischaemic myocardium. Conclusion: These results suggest that there is a tempero–spatial dissociation between the expression of Fas and apoptosis after coronary occlusion. Fas might not directly regulate the apoptosis of cardiomyocytes induced by ischaemia.