Paraffin wax embedded bone marrow trephine blocks used for the extraction of DNA
| PCR results using PBMT DNA samples | ||||||||
|---|---|---|---|---|---|---|---|---|
| IgH clonality | ||||||||
| Patient | Block | DNA | Histology | t(11;14) | t(14;18) | FR2 | FR3 | PCR results using DNA extracted from other tissues |
| FCL, follicle centre lymphoma; MCL, mantle cell lymphoma; N, normal; PBMT, paraffin wax embedded bone marrow trephine. | ||||||||
| 1 | 1 | 1 | N | Negative | Negative | Polyclonal | Polyclonal | No other tissues tested |
| 2 | 2 | 2A,2B | FCL | Negative | Negative | Polyclonal | Polyclonal | t(14;18) not detected in blood or bone marrow aspirate |
| Polyclonal B cell profile generated from blood sample | ||||||||
| 3 | 3 | 3 | FCL | Negative | Positive | Oligoclonal (4 dominant bands) | Polyclonal | t(14;18) detected in biopsy from small intestine |
| 4 | 4 | 4A,4B | MCL | Positive | Negative | Clonal | Clonal | t(11;14) detected in lymph node and peripheral blood |
| 5 | 5 | 5A,5B | MCL | Positive | Negative | Clonal | Clonal | t(11;14) and clonal B cell population detected in lymph node |