Table 1

Conditions for performing the plasminogen activator system and vascular endothelial growth factor (VEGF) ELISAs

ELISA	Dilution	Capture antibody	Incubation	Detection antibody	Conjugate	Standard curve
ELISA, enzyme linked immunosorbent assay; HRP, horseradish peroxidase; PAI, plasminogen activator inhibitor; RT, room temperature; tPA, tissue type plasminogen activator; uPA, urokinase plasminogen activator.
uPA	1/10	Mouse antihuman	Overnight 4°C	Biotinylated antihuman uPA	Streptavidin–HRP	0.1–1.0 ng/ml
tPA	None	Goat anti-tPA	1 hour RT shaking	––	HRP anti-tPA	0.5–10.0
uPAR	1/5	Mouse antihuman	Overnight 4°C	Biotinylated antihuman UpaR	Streptavidin–HRP	0.1–3.0 ng/ml
PAI-1	1/10	Mouse antihuman	Overnight 4°C	Biotinylated antihuman PAI-1	Streptavidin–HRP	0.1–10.0 ng/ml
PAI-2	None	Polyclonal antihuman	2 hours RT	Biotinylated antihuman PAI-2	Streptavidin–HRP	0.5–10 ng/ml
VEGF	None	Mouse antihuman	2 hours RT	––	HRP anti-VEGF	31.2–2000 pg/ml