PCR primers for the amplification of p53 exons 5 to 8
| Exon amplified | Primer sequence |
|---|---|
| *GC clamp sequence (5′-CGC CCG CCG CGC CCC GCG CCC GTC CCG CCG CCC CCG CCC-3′) was added to the 5′ end of exons 5aGC, 5bGC, and 6GC primers; **GC clamp sequence (5′-GCG GGC GGC GCG GGG CGC GGG CAG GGC GGC GGG GGC GGG-3′) was added to the 5′ end of exons 7GC and 8GC primers. | |
| 5aGC | 5′-*GTG TGA CTG CTT GTA GAT-3′ |
| 5a | 5′-CTC TGT CTC CTT CCT CTT-3′ |
| 5bGC | 5′-*GTT CCA CAC CCC CGC CCG GCA-3′ |
| 5b | 5′-GCC CCA GCT GCT CAC CAT CG-3′ |
| 6GC | 5′-*GGA GAG ACG ACA GGG CTG GTT-3′ |
| 6 | 5′-AGT TGC AAA CCA GAC CTC AGG C-3′ |
| 7GC | 5′-**CTA CCT CGC TTA GTG CTC CCT-3′ |
| 7 | 5′-CAC CAT CCA CTA CAA CTA CA-3′ |
| 8GC | 5′-**CTA CCT CGC TTA GTG CTC CCT-3′ |
| 8 | 5′-ATC CTG AGT AGT GGT AAT CT-3′ |