Detection of human papillomavirus 16 (HPV-16) in a background of HPV-18 with GP5+/6+ and with TS 165+/166+ primers
| GP5+/6+ | TS 165+/166+ | |||||
|---|---|---|---|---|---|---|
| SiHa/HeLa | Standard melt Tm | Detailed melt Tm | Comments | Standard melt Tm | Detailed melt Tm | Comments |
| 100/1000 | 80.9°C | 79.9/81.4°C | Double peak | 79.1°C | 78.2°C | Single peak |
| 10/1000 | 81.0°C | 79.9/81.5°C | Double peak | 79.1°C | 78.2°C | Single peak |
| 1/1000 | 81.5°C | 80.1/81.8°C | Double peak | 79.1°C | 78.1°C | Single peak |
| 0.1/1000 | 81.4°C | 80.3°C | Single peak of HPV-18 | No amplification | No amplification | No peak |