Table 1
HinfI digestion of semi-nested PCR products
Mutated
No digestion
Heterozygous
Homozygous/LOH?
Wild-type
*Not visible on a polyacrylamide gel.
LOH, loss of heterozygosity.
189 bp
+
–
–
–
121 bp
–
+
+
+
68 bp
–
+
–
+
54 bp
–
+
+
–
14 bp*
–
+
+
–