Table 1

What to spot: options available

Source of cloneSpotted DNARegion of interestPrimer for cDNA synthesis
SSH, suppression subtractive hybridisation.
Publicly available clones, such as those from the IMAGE consortiumEntire clone or entire plasmidsRegion unknown/unspecifiedRandom hexamers
In house derived—for example, by SSHPCR product from plasmidSpecific regionSpecific primers
Custom made/purchased librariesExpressed sequence tags3′ end of genePoly(T)