Results of the polymerase chain reaction (PCR) assays based on the rpsU gene and the fliC gene
| Species | Source | rpsU PCR | fliC PCR |
|---|---|---|---|
| ATCC, American Type Culture Collection, Atlanta, USA; DSMZ, Deutsche Sammlung von Mikroorganismen, Braunschweig, Germany; IMB, Type collection Institut für Mikrobiologie, Munich, Germany; LMG, Laboratorium Microbiologie Gent Culture Collection, Belgium. | |||
| *Colony forming units/g of organ. | |||
| Burkholderia pseudomallei | ATCC 15682 | 179 bp | 302 bp |
| B pseudomallei | ATCC 23343 | 179 bp | 302 bp |
| B pseudomallei | 6068VIR | 179 bp | 302 bp |
| B thailandensis | ATCC 700388 | 179 bp | 302 bp |
| B. mallei | ATCC 23344 | 179 bp | 302 bp |
| B cepacia | ATCC 25416T | 179 bp | – |
| B vietnamiensis | LMG 10929 | 179 bp | – |
| B gladioli | ATCC 10248 | 179 bp | – |
| B plantarii | LMG 9035 | 179 bp | – |
| B vandii | ATCC 51545 | 179 bp | – |
| Pseudomonas aeruginosa | DSM 1253 | – | – |
| Ps fluorescens | IMB P162 human isolate | – | – |
| Ps stutzeri | NCTC 10450 | – | – |
| Escherichia coli | DSM 301 | – | – |
| Francisella tularensis | ATCC 29684 | – | – |
| Stenotrophomonas maltophilia | IMB P26 human isolate | – | – |
| Staphylococcus aureus | DSM 346 | – | – |
| Staphylococcus epidermidis | DSM 1798 | – | – |
| Spleen mouse 1 (2.09 × 106)* | 179 bp | 302 bp | |
| Spleen mouse 2 (2.21 × 107)* | 179 bp | 302 bp | |
| Spleen mouse 3 (69)* | 179 bp | – | |
| Spleen mouse 4 (1.46 × 108)* | 179 bp | 302 bp | |
| Spleen mouse 5 (1.57 × 109)* | 179 bp | 302 bp | |
| Spleen mouse 6 (2.64 × 108)* | 179 bp | 302 bp | |
| Spleen mouse 7 (8.18 × 108)* | 179 bp | 302 bp | |
| Spleen control mouse | 179 bp | – | |