Measles virus primer and probe sequences
| Primer/Probe | Sequence 5`–3` | Amplicon size |
|---|---|---|
| Measles virus N gene primers and probes were designed based on the following Genbank sequence entries (accession numbers): X16565, S58435, NC_002494, NC_002496, X01999, U03661, U03658, and U03656. Measles virus H gene primers and probes were designed based on the following GenBank sequence entries: X16565, U03649, U03654, U03669, U03660, U03671, U03667, Z80793, AF045204, Z66517, and Z80816. Measles virus F-gene primers and probes were designed based on the following Genbank sequence entries: X16565, U03655, U03666, U03648, U03662, U08146, U03657, U03651, U03659, AJ133108, X16567. | ||
| N1 forward | 5` TCA GTA GAG CGG TTG GAC CC 3` | |
| N1 reverse | 5` GGC CCG GTT TCT CTG TAG CT 3` | 150 bp |
| N2 forward | 5` GAG TCG AGG AGA AGC CAG GG 3` | |
| N2 reverse | 5` GCT GGA CTC CGA TGC AGT GT 3` | 120 bp |
| H1 forward | 5` TTC ATC GGG CAG CCA TCT AC 3` | |
| H1 reverse | 5` CTC TGA GGT GTC CTC AGG CC 3` | 150 bp |
| H2 forward | 5` TGG GCA CCA TTG AAG GAT AA 3` | |
| H2 reverse | 5` AAC CGT GTG TGA TCA ATG GC 3` | 120 bp |
| F1 forward | 5` TGA CTC GTT CCA GCC ATC AA 3` | |
| F1 reverse | 5` TGG GTC ATT GCA TTA AGT GCA 3` | 150 bp |
| F2 forward | 5` CCC ACC GGT CAA ATC CAT T 3` | |
| F2 reverse | 5` CCC TCG TGC AGT TAT TGA GGA 3` | 150 bp |
| GAPDH 1 | 5` GAA GGT GAA GGT CGG AGT 3` | |
| GAPDH 2 | 5` GAA GAT GGT GAT GGG ATT TC 3` | 226 bp |
| N1 probe | 5` CAA ACA GAG TCG AGG AGA AGC CAG GGA 3` | |
| H1 probe | 5` CCG CAG AGA TCC ATA AAA GCC TCA GCA C 3` | |
| F1 probe | 5` CTG CAC GAG GGT AGA GAT CGC AGA ATA CAG 3` | |
| GAPDH probe | 5` CCG ACT CTT GCC CTT CGA AC 3` | |