Table 1

Immunophenotype, clonality, and subtype of the cases of acute leukaemia investigated

	Immunoreactivity				Monoclonality
No.	MPO/Lys	TdT	CD10/CD20/CD79a	CD3/βF1	IgH	TCR-γ	Primary diagnosis	Probable lineage on the basis of PCR
Cases were recorded as immunoreactive if more than 10% of the blast cells were positive. AL, acute leukaemia; ALL, acute lymphoblastic leukaemia; AML, acute myelogenous leukaemia; B-ALL, B cell ALL; IgH, immunoglobulin heavy chain; MPO, myeloperoxidase; Lys, lysozyme; PCR, polymerase chain reation; T-ALL, T cell ALL; TCR-γ, T cell receptor γ, TdT, terminal deoxynucleotidyl transferase; u-ALL, unclassified ALL.
1			CD10/CD20		+		c-ALL
2			CD10/CD20	βF1	+		c-ALL
3			CD10		+		c-ALL
4		TdT	CD10		+		c-ALL
5		TdT	CD10/CD79a		+		c-ALL
6		TdT	CD10/CD79a	βF1	+		c-ALL
7		TdT	CD10	βF1	+		c-ALL
8		TdT	CD10/CD79a		+	+	c-ALL	ALL, bilinear genotype?
9		TdT	CD10/CD20		+		c-ALL
10		TdT	CD10/CD79a	CD3			c-ALL
11		TdT	CD10/CD79a		+		c-ALL
12		TdT	CD10/CD20		+		c-ALL
13		TdT	CD10/CD79a		+		c-ALL
14		TdT	CD10/CD79a		+		c-ALL
15		TdT	CD10/CD79a		+		c-ALL
16		TdT	CD10/CD79a		+		c-ALL
17			CD10/CD20		+		c-ALL
18		TdT	CD10			+	c-ALL	T-ALL
19			CD10	CD3/βF1			c-ALL
20		TdT	CD10/CD20/CD79a	βF1	+		c-ALL
21		TdT	CD10/CD79a		+		c-ALL
22		TdT	CD10		+		c-ALL
23		TdT	CD10/CD79a		+		c-ALL
24				CD3/βF1		+	T-ALL
25		TdT		βF1		+	T-ALL
26		TdT		CD3			T-ALL
27		TdT		βF1			T-ALL
28		TdT		βF1		+	T-ALL
29						+	T-ALL
30		TdT		βF1	+		T-ALL	ALL, B cell genotype?
31			CD20/CD79a		+		B-ALL
32			CD20/CD79a	CD3	+		u-ALL	ALL, B cell genotype
33					+		u-ALL	ALL, B cell genotype
34			CD20		+		u-ALL	ALL, B cell genotype
35		TdT			+	+	u-ALL	ALL, bilinear genotype
36	MPO	TdT			+		AL, biphenotypic
37	MPO		CD20	βF1			AL, biphenotypic
38	MPO	TdT	CD79a		+		AL, biphenotypic	B cell type
39	MPO				+		AML-M1	B cell genotype?
40	MPO						AML-M1
41	MPO						AML-M1
42	MPO						AML-M1
43	MPO/Lys						AML-M4
44	MPO/Lys						AML-M4
45	MPO						AML-M4
46	MPO						AML-M4
47	MPO						AML-M4
48	MPO/Lys				+		AML-M5	B-cell genotype ?